Article
Genetic characterization of dengue virus type 1 isolated in Brunei in 2005-2006.
The Journal of general virology - 1 Mar 2009
Osman Osmali, Fong Mun Yik, Sekaran Shamala Devi
Abstract excerpt
The full-length genomes of two DENV-1 viruses isolated during the 2005-2006 dengue incidents in Brunei were sequenced. Twenty five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the genome. The amplified PCR products were sent to a commercial laboratory for sequencing and the nucleotides and the deduced amino acids were...
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