Article
High-throughput bioluminescence-based mutant screening strategy for identification of bacterial virulence genes.
Applied and environmental microbiology - 1 Apr 2009
Karsi Attila, Gülsoy Nagihan, Corb Erin, Dumpala Pradeep R, Lawrence Mark L
Abstract excerpt
A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. M...
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