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Utility of Real-time PCR for the detection of<i>Paracoccidioides brasiliensis</i>DNA in the diagnosis of imported paracoccidioidomycosis

1 Dec 2009

Abstract excerpt

An increase in immigration from endemic regions has resulted in a number of cases of paracoccidioidomycosis (PCM) being imported into Spain. A molecular diagnostic technique based on real-time PCR was developed for the detection of Paracoccidioides brasiliensis DNA in both culture and patients' clinical samples. A Molecular Beacon probe was used, labelled with FAM and directed at the ITS1 region of ribosomic DNA....

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PMID: 19184775
DOI: 10.3109/13693780802713208

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