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Molecular Identification ofParacoccidioides brasiliensisby PCR Amplification of Ribosomal DNA

1 Aug 2000

Abstract excerpt

We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and...

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PMID: 10921990
DOI: 10.1128/jcm.38.8.3106-3109.2000

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