Article
The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in Saccharomyces cerevisiae.
The Journal of cell biology - 1 Oct 1991
McCaffrey M, Johnson J S, Goud B, Myers A M, Rossier J, Popoff M R, Madaule P, Boquet P
Abstract excerpt
In Saccharomyces cerevisiae the ras-related protein Rho1p is essentially the only target for ADP-ribosylation by exoenzyme C3 of Clostridium botulinum. Using C3 to detect Rho1p in subcellular fractions, Rho1p was found primarily in the 10,000 g pellet (P2) containing large organelles; small amounts also were detected in the 100,000 g pellet (P3), and cytosol. When P2 organelles were separated in sucrose density...
Topics
- Blotting, Western
- Botulinum Toxins
- Centrifugation, Density Gradient
- Fungal Proteins
- GTP-Binding Proteins
- Golgi Apparatus
- Immune Sera
- Membrane Proteins
- Microscopy, Fluorescence
- Mutation