Article
Functional domains of Bacillus thuringiensis insecticidal crystal proteins. Refinement of Heliothis virescens and Trichoplusia ni specificity domains on CryIA(c).
The Journal of biological chemistry - 25 Sept 1991
Ge A Z, Rivers D, Milne R, Dean D H
Abstract excerpt
Insecticidal crystal proteins (delta-endotoxins), CryIA(a) and CryIA(c), from Bacillus thuringiensis are 82% homologous. Despite this homology, CryIA(c) was determined to have 10-fold more insecticidal activity toward Heliothis virescens and Trichoplusia ni than CryIA(a). Reciprocal recombinations between these two genes were performed by the homolog-scanning technique. The resultant mutants had different...
Topics
- Bacillus thuringiensis
- Bacillus thuringiensis Toxins
- Bacterial Proteins
- Bacterial Toxins
- Electrophoresis, Polyacrylamide Gel
- Endotoxins
- Hemolysin Proteins
- Mutagenesis
- Mutation
- Pest Control, Biological