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Multiplex real‐time PCR for the simultaneous detection of herpes simplex virus, human herpesvirus 6, and human herpesvirus 7

1 Jan 2009

Abstract excerpt

A simultaneous detection system to quantify HSV, HHV-6, and HHV-7 DNA via multiplex real-time PCR using different fluorochromes was developed. The minimum quantitative level established via this multiplex assay was four copies per reaction for HSV type 1, four copies for HHV-6, and three copies for HHV-7, respectively. The dynamic range encompassed at least six orders of magnitude. The system was specific and...

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PMID: 19161554
DOI: 10.1111/j.1348-0421.2008.00090.x

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