Article
[Significance of IGS1, ITS and D1/D2 regions in molecular identification of Trichosporon species and genotype of T. asahii in China].
Zhonghua yi xue za zhi - 2 Dec 2008
Xia Zhi-Kuan, Yang Rong-Ya, Wang Wen-Ling, Ao Jun-Hong, Wang Cong-Min, Zhu He
Abstract excerpt
OBJECTIVE: To compare the sensitivity and specificity in molecular identification in different DNA regions of Trichosporon species and to study the genotype of T. asahii isolated from clinical specimens in China. METHODS: DNA was extracted from the cells of all experimental strains by using a method of glass bead method. The D1/D2, ITS and IGS1 regions were amplified by PCR with specific primers, the PCR products...
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