Article
The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli.
European journal of biochemistry - 1 Oct 1991
Lee C Y, Szittner R B, Meighen E A
Abstract excerpt
The lux genes required for light expression in the luminescent bacterium Photobacterium leiognathi (ATCC 25521) have been cloned and expressed in Escherichia coli and their organization and nucleotide sequence determined. Transformation of a recombinant 9.5-kbp chromosomal DNA fragment of P. leiognathi into an E. coli mutant (43R) gave luminescent colonies that were as bright as those of the parental strain....
Topics
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Codon
- DNA, Recombinant
- Escherichia coli
- Gene Expression
- Luciferases
- Luminescent Measurements