SSCP analysis of cDNA markers provides a dense linkage map of the Aedes aegypti genome.

doi:10.1093/genetics/158.2.715

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Article

SSCP analysis of cDNA markers provides a dense linkage map of the Aedes aegypti genome.

Genetics - 1 Jun 2001

Fulton R E, Salasek M L, DuTeau N M, Black W C

Abstract excerpt

An intensive linkage map of the yellow fever mosquito, Aedes aegypti, was constructed using single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleotide polymorphisms (SNPs). A total of 94 A. aegypti cDNAs were downloaded from GenBank and primers were designed to amplify fragments <500 bp in size. These primer pairs amplified 94 loci, 57 (61%) of which segregated in a...

Topics

Aedes
Animals
Base Sequence
Chromosome Mapping
Crosses, Genetic
DNA, Complementary
Expressed Sequence Tags
Genetic Linkage
Genetic Markers
Genome
Genotype
Microsatellite Repeats
Molecular Sequence Data
Polymorphism, Single-Stranded Conformational
Sequence Homology, Nucleic Acid

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Identifiers

PMID: 11404335
DOI: 10.1093/genetics/158.2.715
PMCID: PMC1461678

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