Article
Differentiation of Cherry leaf roll virus isolates from various host plants by immunocapture-reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations.
Journal of virological methods - 1 May 2009
Buchhop Jutta, von Bargen Susanne, Büttner Carmen
Abstract excerpt
A restriction fragment length polymorphism assay (RFLP) was developed to differentiate Cherry leaf roll virus (CLRV) isolates according to phylogenetic clades by examining restriction patterns from partial 3' non-coding region (NCR) genomic fragments (approx. 420bp). The 3' NCR fragment from 43 CLRV isolates belonging to different phylogenetic groups were compared after restriction analysis with the endonucleases...
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