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Rapid Detection of <i>bla</i> <sub>KPC</sub> Carbapenemase Genes by Real-Time PCR

10 Jul 2008

Abstract excerpt

Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Klebsiella pneumoniae carbapenemase (bla(KPC)) enzymes are among the most common beta-lactamases described. In this study, we report the development and validation of a real-time PCR (q-PCR) assay for the detection of bla(KPC) genes using TaqMan chemistry. The q-PCR amplification of bla(KPC) DNA was linear over 7 log dilutions (r(2)...

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Identifiers

PMID: 18614657
DOI: 10.1128/jcm.00661-08

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