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Colon Cancer Stem Cells

1 Nov 2008

Abstract excerpt

This unit describes protocols for working with colon cancer stem cells. To work with these cells one must start by generating single-cell suspensions from human colon cancer tissue. These cell suspensions are sorted using flow cytometry-assisted cell sorting to fractionate the cells into tumor-initiating and nontumor-initiating subsets. Once the cells have been fractionated, they must be functionally tested to...

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PMID: 19016431
DOI: 10.1002/9780470151808.sc0301s7

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