Article
Molecular organization and dynamics of the outer membrane of Salmonella thyphimurium mutant strains detected by frequency domain fluorometry.
Archives of biochemistry and biophysics - 1 May 1991
Bismuto E, Sirangelo I, Adinolfi A, Galdiero F, Tufano M A, Sommese L, Irace G
Abstract excerpt
The fluorescence decay of 1,6-diphenyl-1,3,5-hexatriene (DPH) in the outer membrane bilayer of two mutant strains of Salmonella thyphimurium, i.e., SH 5014 and SH 6261, at different temperatures was analyzed in terms of continuous Lorentzian lifetime distributions. The results were compared with those obtained for the free fluorophore in an isotropic nonviscous solvent. The incorporation of DPH in the outer...
Topics
- Bacterial Outer Membrane Proteins
- Cell Membrane
- Diphenylhexatriene
- Mutation
- Salmonella typhimurium
- Spectrometry, Fluorescence
- Thermodynamics