Article
One-dimensional isoelectric focusing and immunoblotting of bovine major histocompatibility complex (BoLA) class I molecules and correlation with class I serology.
Animal genetics - 1 Jan 1991
Viuff B, Ostergård H, Aasted B, Kristensen B
Abstract excerpt
One-dimensional isoelectric focusing followed by immunoblotting and development of the immunoblots with the monoclonal antibody HC-10, raised against denatured HLA class I heavy chains, was used to demonstrate biochemical variation in cattle MHC (BoLA) class I molecules. The bands obtained correlated well with BoLA-A specificities. Two or three bands were identified for the specificities w7, w8, w16, w18, w21,...
Topics
- Animals
- Cattle
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Histocompatibility Antigens Class I
- Immunoblotting
- Isoelectric Focusing
- Major Histocompatibility Complex
- Precipitin Tests
- Serotyping