Article
Major histocompatibility complex (MHC) protein analysis by optimised two-dimensional electrophoretic methods.
Electrophoresis - 1 Feb 1990
Fernandez N, Labeta M, Kurpisz M, Nakatsuji T, Sachs J, Festenstein H
Abstract excerpt
Human histocompatibility molecules HLA-Class I and Class II (DR, DQ, DP) were analysed using three two-dimensional protocols: nonequilibrium pH gradient electrophoresis (NEPHGE), isoelectric focusing-acidic gradient (IEF-AG) and isoelectric focusing-basic gradient (IEF-BG). The three methods differ in their carrier ampholyte combinations and electrophoretic conditions. They provide different pH gradients and...
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