Article
Major histocompatibility complex (MHC) protein analysis by optimised two-dimensional electrophoretic methods.
Electrophoresis - 1 Feb 1990
Fernandez N, Labeta M, Kurpisz M, Nakatsuji T, Sachs J, Festenstein H
Abstract excerpt
Human histocompatibility molecules HLA-Class I and Class II (DR, DQ, DP) were analysed using three two-dimensional protocols: nonequilibrium pH gradient electrophoresis (NEPHGE), isoelectric focusing-acidic gradient (IEF-AG) and isoelectric focusing-basic gradient (IEF-BG). The three methods diff...
Topics
- Alleles
- Antibodies, Monoclonal
- Electrophoresis, Gel, Two-Dimensional
- HLA Antigens
- HLA-D Antigens
- HLA-DP Antigens
- HLA-DR Antigens
- Humans
- Hydrogen-Ion Concentration
- Isoelectric Focusing
- Polymorphism, Genetic
- Precipitin Tests