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Matrix Metalloproteinases

1 Sept 2008

Abstract excerpt

Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods--cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on alpha2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase...

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PMID: 18819088
DOI: 10.1002/0471143030.cb1008s40

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