Article
Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens.
Memorias do Instituto Oswaldo Cruz - 1 Jun 2008
Gouveia Ana Cláudia Carvalho, Eisenach Kathleen D, Vinhas Solange Alves, Ribeiro Fabiola Karla Corrêa, Peres Renata Lyrio, Dietze Reynaldo, Hadad David Jamil, Palaci Moisés
Abstract excerpt
We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC 12B vials. At a growth index (GI) > or=30, 1.0 ml of the 12B broth was removed, stored, and assayed with PCR. Molecular results were compared to those obtained by phenotypic identification...
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