Nested PCRs and sequencing of nuclear ITS‐rDNA fragments detect three <i>Leishmania</i> species of gerbils in sandflies from Iranian foci of zoonotic cutaneous leishmaniasis

Abstract excerpt

OBJECTIVE: To identify and understand the natural transmission cycles of Leishmania in Iranian sandflies. METHOD: Nested PCR protocols were developed to amplify two regions of the ribosomal RNA amplicon of Leishmania (ITS1-5.8S rRNA gene, and a microsatellite DNA region of ITS2), which were species-specific by DNA sequence or fragment size. RESULTS: The PCR assays detected in Iranian sandflies not only Leishmania...

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