Article
Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis.
Molecular genetics and genomics : MGG - 1 Aug 2008
Park Dong-Soo, Song Min-Young, Park Soo-Kwon, Lee Sang-Kyu, Lee Jong-Hee, Song Song-Yi, Eun Moo Young, Hahn Tae-Ryong, Sohn Jae-Keun, Yi Gihwan, Nam Min-Hee, Jeon Jong-Seong
Abstract excerpt
During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in...
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