Article
Analysis of Rhizobium meliloti nodulation mutant WL131: novel insertion sequence ISRm3 in nodG and altered nodH protein product.
Journal of bacteriology - 1 May 1991
Ogawa J, Brierley H L, Long S R
Abstract excerpt
Nodulation (nod) genes are required for invasion of legumes by Rhizobium bacteria. Mutant WL131 is a derivative of 102F51 that has a severe Nod- phenotype on alfalfa. Upon examination of the extended DNA region containing host-specific nodulation genes nodFEG and nodH, we found that the nodG gene of WL131 bears a novel insertion sequence, ISRm3. Complementation studies implied, however, that the phenotype on...
Topics
- Blotting, Southern
- DNA Transposable Elements
- DNA, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Phenotype
- Restriction Mapping
- Rhizobium
- Symbiosis