Article
A TaqI PCR-RFLP detecting a novel SNP in exon 2 of the bovine POU1F1 gene.
Biochemical genetics - 1 Aug 2008
Pan Chuanying, Lan Xianyong, Chen Hong, Guo Yikun, Shu Jianhong, Lei Chuzhao, Wang Xinzhuang
Abstract excerpt
PCR-SSCP and DNA sequencing methods were applied to reveal three novel single nucleotide polymorphisms (SNPs) in exon 2 of the POU1F1 gene in 963 Chinese cattle belonging to eight breeds. Among them, a silent SNP (NM_174579:c.545G > A) detected by TaqI endonuclease is described. Frequencies of the POU1F1-G allele varied from 0.685 to 1.000. The association of TaqI polymorphism with growth traits was analyzed in...
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