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Development and optimization of an in vitro chloroplastic protein import assay using recombinant proteins.

Plant physiology and biochemistry : PPB - 1 Jan 2000

Inoue Hitoshi, Ratnayake R M Udayangani, Nonami Hiroshi, Akita Mitsuru

Abstract excerpt

The in vitro protein import experiment is one of the most important techniques for determining protein localization. For chloroplastic proteins, proteins of interest are incubated with isolated chloroplasts in the presence of energy sources. Radio-labeled proteins synthesized either in vitro or in vivo have been widely used as substrate proteins. Here we report our development of the protein import assay system...

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PMID: 18417353
DOI: 10.1016/j.plaphy.2008.02.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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