Article
Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12.
Proceedings of the National Academy of Sciences of the United States of America - 1 Dec 1991
Nakahigashi K, Nishimura K, Miyamoto K, Inokuchi H
Abstract excerpt
Mutations in the visA gene of Escherichia coli cause the mutant bacteria to die upon illumination with visible light. We confirmed genetically that the visA gene is a structural gene for ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1). Since other mutations in the genes involved in the biosynthesis of heme can cure the photosensitivity, the light-induced cell death appears to be brought about by the...
Topics
- Anaerobiosis
- Bacteriophage lambda
- Catalase
- Chromosome Deletion
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Ferrochelatase
- Genes, Bacterial
- Genotype
- Light