Article
Two-dimensional DNA gel electrophoresis mapping: a novel approach to diversity analysis of bacterial communities in environmental soil.
Journal of bioscience and bioengineering - 1 Feb 2008
Liu Guohua, Amemiya Takashi, Itoh Kiminori
Abstract excerpt
The diversity analysis of bacteria is useful for the environmental assessment of soil. Traditional molecular-based methods such as denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analysis achieve a low-resolution display of bacterial DNA fragments on a gel. To improve the resolution, a novel two-dimensional DNA gel electrophoresis (2-DGE) method was...
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