Article
Amplified functional DNA restriction analysis to determine catechol 2,3-dioxygenase gene diversity in soil bacteria.
Journal of microbiological methods - 1 Dec 2003
Junca Howard, Pieper Dietmar H
Abstract excerpt
To determine phylogenetic diversity of a functional gene from strain collections or environmental DNA amplifications, new and fast methods are required. Catechol 2,3-dioxygenase (C23O) subfamily I.2.A genes, known to be of crucial importance for aromatic degradation, were used as a model to adapt the amplified ribosomal DNA restriction analysis to functional genes. Sequence data of C23O genes from 13 reference...
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