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Rapid and Reliable Method for Quantification of <i>Mycobacterium paratuberculosis</i> by Use of the BACTEC MGIT 960 System

12 Apr 2007

Abstract excerpt

A simple method for the enumeration of viable Mycobacterium paratuberculosis cells was developed and evaluated using the MGIT 960 culture system. For each of 12 M. paratuberculosis strains isolated from either cattle or humans, single-cell suspensions of M. paratuberculosis cells were adjusted to an optical density at 600 nm of 1.00 (10(7.6) to 10(8.2) cells/ml), and serial dilutions were prepared. Standard...

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PMID: 17428943
DOI: 10.1128/jcm.02616-06

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