Article
Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions.
Haematologica - 1 Apr 2008
Randen Ingrid, Sørensen Kirsten, Hauge Reidun, Dahlberg Anine B, Mirlashari Mohammad R, Thompson Keith M, Kjeldsen-Kragh Jens
Abstract excerpt
Alloimmunization is a common phenomenon after transfusion, with an estimated incidence of 0.5% increasing to 20-60% in chronically transfused patients. In recently transfused patients, serological typing can be hampered by mixed field agglutination. We established RT-PCR methods for RHD, RHC/c and RHE/e typing using mRNA from reticulocytes. Molecular typing was performed soon after 51 separate mismatched...
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