Article
High-throughput SNP discovery through deep resequencing of a reduced representation library to anchor and orient scaffolds in the soybean whole genome sequence.
BMC genomics - 15 Jan 2010
Hyten David L, Cannon Steven B, Song Qijian, Weeks Nathan, Fickus Edward W, Shoemaker Randy C, Specht James E, Farmer Andrew D, May Gregory D, Cregan Perry B
Abstract excerpt
BACKGROUND: The Soybean Consensus Map 4.0 facilitated the anchoring of 95.6% of the soybean whole genome sequence developed by the Joint Genome Institute, Department of Energy, but its marker density was only sufficient to properly orient 66% of the sequence scaffolds. The discovery and genetic mapping of more single nucleotide polymorphism (SNP) markers were needed to anchor and orient the remaining genome...
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