Synthesis, accumulation, and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the periplasmic trehalase.

Abstract excerpt

It has been reported previously that Escherichia coli K-12 carries an amber mutation that prevents osmotic stress-dependent accumulation of trehalose (M. L. Rod, K. Y. Alam, P. R. Cunningham, and D. P. Clark, J. Bacteriol. 170:3601-3610, 1988). We report that E. coli K-12 and W1485 (sup0) accumulated trehalose but that they required a higher osmotic strength in the growth medium than that required by their sup+...

Topics

• Escherichia coli
• Genes, Bacterial
• Genes, Suppressor
• Glucosyltransferases
• Kinetics
• Mutation
• Operon
• Osmolar Concentration
• Trehalase
• Trehalose