Article
Novel x-type high-molecular-weight glutenin genes from Aegilops tauschii and their implications on the wheat origin and evolution mechanism of Glu-D1-1 proteins.
Genetics - 1 Jan 2008
Zhang Yanzhen, Li Xiaohui, Wang Aili, An Xueli, Zhang Qian, Pei Yuhe, Gao Liyan, Ma Wujun, Appels Rudi, Yan Yueming
Abstract excerpt
Two new x-type high-molecular-weight glutenin subunits with similar size to 1Dx5, designated 1Dx5*t and 1Dx5.1*t in Aegilops tauschii, were identified by SDS-PAGE, RP-HPLC, and MALDI-TOF-MS. The coding sequences were isolated by AS-PCR and the complete ORFs were obtained. Allele 1Dx5*t consists of 2481 bp encoding a mature protein of 827 residues with deduced Mr of 85,782 Da whereas 1Dx5.1*t comprises 2526 bp...
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