Expression of the fluorescence markers DsRed and GFP fused to a nuclear localization signal in the arbuscular mycorrhizal fungus <i>Glomus intraradices</i>

Abstract excerpt

Here, arbuscular mycorrhizal (AM) fungi were monitored in vivo introducing the fluorescent reporters DsRed and GFP (green fluorescent protein) in Glomus intraradices using a biolistic approach and Agrobacterium tumefaciens-mediated transformation. Both reporter genes were fused to the nuclear localization signal of the Aspergillus nidulans transcription factor StuA to target fluorescence to nuclei. Expression of...

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