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Quantification of the arbuscular mycorrhizal fungus <i> Glomus intraradices</i> in host tissue using real‐time polymerase chain reaction

30 Jan 2004

Abstract excerpt

• A rapid method to quantify the colonization of the arbuscular mycorrhizal fungus (AMF) Glomus intraradices in planta using quantitative real-time polymerase chain reaction (qRT-PCR) technique. • Specific PCR primers for the fungus (28S rDNA sequence) and host root tissue (chitinase and chalcone synthase gene) were developed and their respective specificity determined. • The plant specific primers for...

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PMID: 33873725
DOI: 10.1046/j.1469-8137.2004.00975.x

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