Article
Single-nucleotide primer extension assay for detection and sequence typing of "Dehalococcoides" spp.
Applied and environmental microbiology - 1 Jan 2008
Nikolausz Marcell, Chatzinotas Antonis, Palatinszky Márton, Imfeld Gwenaël, Martinez Paula, Kästner Matthias
Abstract excerpt
A single-nucleotide primer extension (SNuPE) assay in combination with taxon-specific 16S rRNA gene PCR analysis was developed for the detection and typing of populations of the genus "Dehalococcoides". The specificity of the assay was evaluated with 16S rRNA gene sequences obtained from an isolate and an environmental sample representing two Dehalococcoides subgroups, i.e., the Cornell and the Pinellas...
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