Article
Development of a hierarchical oligonucleotide primer extension assay for the qualitative and quantitative analysis of Cylindrospermopsis raciborskii subspecies in freshwater.
Microbes and environments - 1 Jan 2010
Wu Jer-Horng, Hsu Mao-Hsuang, Hung Ching-Hui, Tseng I-Cheng, Lin Tsair-Fuh
Abstract excerpt
A newly-developed molecular method, hierarchical oligonucleotide primer extension (HOPE), was used to analyze various groups within the species Cylindrospermopsis raciborskii. PCR-amplified internally transcribed spacer sequences of 16S-23S from C. raciborskii in reservoir samples of Taiwan and Kinmen were examined. One of eight sequevars in the clone libraries was closely related to strains obtained from the...
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