Article
Quantitative discrimination of 16 S rRNA genes of Dehalococcoides species by MagSNiPer, a quantitative single-nucleotide-polymorphism genotyping method.
Biotechnology and applied biochemistry - 1 Oct 2008
Yohda Masafumi, Kobayashi Ai, Ohishi Shoji, Ebisawa Maiko, Sakihara Sakari, Kakihara Fumiko, Tojo Yuriko, Tajima Hideji, Nishimura Minoru
Abstract excerpt
Previously we developed MagSNiPer, an SNP (single nucleotide polymorphism) genotyping method. In the present paper we show development of an automated system for MagSNiPer, namely MagSNiPer Station, and its application for quantitative discrimination of Dehalococcoides species, which perform anaerobic dechlorination of chloroethenes. MagSNiPer Station is equipped with a thermal cycler, a tip stand, a...
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