Article
Effect of new SNP within bovine prolactin gene enhancer region on expression in the pituitary gland.
Biochemical genetics - 1 Oct 2007
Brym P, Malewski T, Starzyński R, Flisikowski K, Wójcik E, Ruść A, Zwierzchowski L, Kamiński S
Abstract excerpt
A new single nucleotide polymorphism was revealed using PCR-SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A-->G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR-RFLP genotyping. The application of real-time PCR revealed that the prolactin gene expression level in the pituitary...
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