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DNA Pyrosequencing-Based Bacterial Pathogen Identification in a Pediatric Hospital Setting

26 Jul 2007

Abstract excerpt

Sole reliance on biochemical methods can limit the clinical microbiology laboratory's ability to identify bacterial pathogens. This study describes the incorporation of DNA pyrosequencing-based identification for routine pathogen identification of atypical clinical isolates in a large children's hospital. The assay capitalized on the highly conserved nature of 16S rRNA genes by positioning amplification and...

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PMID: 17652476
DOI: 10.1128/jcm.00630-07

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