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Article

Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service

17 Jul 2003

Abstract excerpt

The aim of the present study was to develop a broad-range PCR based on bacterial 16S rDNA for use in the routine diagnostic clinical microbiology service. The optimization and validation of the assay for use on clinical specimens from normally sterile sites is described, and preliminary results are reported on the use of the assay in the clinical diagnosis of bacterial infection in 382 paediatric specimens over a...

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PMID: 12867563
DOI: 10.1099/jmm.0.05213-0

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