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Article

[Prokaryotic expression and purification of SRG4, a novel mouse spermatogenesis gene].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Aug 2007

Xing Xiao-wei, Yuan Hong, Wang Wei

Abstract excerpt

AIM: To express SRG4, a novel mouse spermatogenesis gene in E.coli and purify its fusion protein. METHODS: RT-PCR was used to amplify the 586 bp fragment that was located in SRG4 C-end and included a Sad1_UNC like domain. The PCR products were cloned into pUCm-T vectors and sequenced. Then the cD...

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PMID: 17618558

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17618558-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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