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Article

Trypanosoma cruzi: subtractive hybridization as a molecular strategy to generate new targets to distinguish groups and hybrids.

Experimental parasitology - 1 Oct 2007

Toma Helena K, Yamada-Ogatta Sueli F, Brandão Adeilton, Krieger Marco A, Goldenberg Samuel, Fernandes Octavio

Abstract excerpt

RAPD analysis and sequences of the mini-exon and ribosomal genes show that Trypanosoma cruzi can be clustered into two phylogenetic groups-T. cruzi I and II. Herein, the Representational Difference Analysis (RDA) method was used, providing new targets specific for each group. After three rounds of RDA hybridizing F strain (tester) with Y strain (driver) and vice-versa, an F-specific (F#30) and Y-specific (Y#22)...

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PMID: 17597613
DOI: 10.1016/j.exppara.2007.04.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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