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A high‐throughput strategy for protein profiling in cell microarrays using automated image analysis

1 Jun 2007

Abstract excerpt

Advances in antibody production render a growing supply of affinity reagents for immunohistochemistry (IHC), and tissue microarray (TMA) technologies facilitate simultaneous analysis of protein expression in a multitude of tissues. However, collecting validated IHC data remains a bottleneck problem, as the standard method is manual microscopical analysis. Here we present a high-throughput strategy combining IHC...

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PMID: 17549799
DOI: 10.1002/pmic.200700199

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