Article
Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17.
Biochemical and biophysical research communications - 8 Jun 2007
Choi Ki Young, Kim Dockyu, Chae Jong-Chan, Zylstra Gerben J, Kim Eungbin
Abstract excerpt
The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the...
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