Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site.

Abstract excerpt

Endoribonuclease RNase E has an important role in the processing and degradation of bacteriophage T4 and Escherichia coli mRNAs. We have undertaken a mutational analysis of the -71 RNase E processing site of T4 gene 32. A series of mutations were introduced into a synthetic T4 sequence cloned on a plasmid, and their effects on processing were analyzed in vivo. The same mutations were transferred into T4 by...

Topics

• Base Composition
• Base Sequence
• Endoribonucleases
• Escherichia coli
• Genes, Viral
• Molecular Sequence Data
• Mutation
• Plasmids
• RNA Processing, Post-Transcriptional
• RNA, Messenger
• RNA, Viral