Article
Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method.
International journal of food microbiology - 10 May 2007
Hassan Abdulwahed Ahmed, Akineden Omer, Kress Claudia, Estuningsih Sri, Schneider Elisabeth, Usleber Ewald
Abstract excerpt
The gene encoding the 16S rRNA of Enterobacter (E.) sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between E. sakazakii and other Enterobacteriaceae within the hypervariable regions V1, V2, and V3, respectively, were used to develop two PCR methods...
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