Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable <i>Mycobacterium paratuberculosis</i> Bacteria within 48 Hours

Abstract excerpt

The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based...

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