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Development of a Peptide-Mediated Capture PCR for Detection of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in Milk

30 Oct 2002

Abstract excerpt

Based on phage display technology, a peptide-mediated magnetic separation technique was developed to facilitate selective isolation of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) from bulk milk of naturally infected dairy herds. Nine recombinant bacteriophages binding to M. paratuberculosis were isolated from a commercial phage-peptide library encoding random 12-mer peptides. Nucleotide...

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PMID: 12409405
DOI: 10.1128/jcm.40.11.4244-4250.2002

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