Article
[Sequence analysis of HLA-B*4061 allele newly found].
Zhongguo shi yan xue ye xue za zhi - 1 Dec 2006
Zhang Wei, Lü Qin-Feng, Wang Wei, Han Zhe-Dong, Zhu Fa-Ming, Yan Li-Xing
Abstract excerpt
The aim of this study was aimed to investigate the molecular genetic basis for a novel HLA allele, HLA-B*4061, in Chinese population. DNA was extracted from whole blood by salting-out method. The HLA-B exons 1 - 8 of the proband was amplified and the amplified product was cloned using TOPO TA cloning sequencing kit to split the two alleles apart. Both strands of exons 2, 3 and 4 of chosen colonies were...
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