Article
Detection of the apoB-3500 mutation (glutamine for arginine) by gene amplification and cleavage with MspI.
Journal of lipid research - 1 Jul 1991
Hansen P S, Rüdiger N, Tybjaerg-Hansen A, Faergeman O, Gregersen N
Abstract excerpt
A single primer-template mismatch 2 bp from the apoB-3500 (G to A) mutation permits introduction of a cleavage site for MspI (C/CGG) in normal alleles but not in mutant alleles (CCAG). After amplification, cleavage, and polyacrylamide gel electrophoresis, normal and mutant alleles could be unambi...
Topics
- Apolipoproteins B
- Arginine
- Base Sequence
- Deoxyribonuclease HpaII
- Deoxyribonucleases, Type II Site-Specific
- Gene Amplification
- Glutamine
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation