Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of the apoB-3500 mutation (glutamine for arginine) by gene amplification and cleavage with MspI.

Journal of lipid research - 1 Jul 1991

Hansen P S, Rüdiger N, Tybjaerg-Hansen A, Faergeman O, Gregersen N

Abstract excerpt

A single primer-template mismatch 2 bp from the apoB-3500 (G to A) mutation permits introduction of a cleavage site for MspI (C/CGG) in normal alleles but not in mutant alleles (CCAG). After amplification, cleavage, and polyacrylamide gel electrophoresis, normal and mutant alleles could be unambiguously distinguished. We constructed a positive (homozygous mutant) standard by site-directed mutagenesis. A negative...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Apolipoproteins B
Share to Arginine
Share to Base Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Deoxyribonucleases, Type II Site-Specific

Share to Gene Amplification

Share to Molecular Sequence Data

Share to Mutagenesis, Site-Directed

Share to Mutation

Source

Identifiers

PMID: 1719111

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection of the apoB-3500 mutation (glutamine for arginine) by gene amplification and cleavage with MspI.PMID 1719111Read ↗

Rapid detection of 3500Q and 3531 mutations and MspI polymorphism in exon 26 at the apolipoprotein B gene.Semantic proximity Read ↗

Apolipoprotein B(Arg3500----Gln) allele specific polymerase chain reaction: large-scale screening of pooled blood samples.Semantic proximity Read ↗

Detection of the apoB-3500 mutation in a Russian family with coronary heart disease.Semantic proximity Read ↗

Familial defective apolipoprotein B-100: haplotype analysis of the arginine(3500)----glutamine mutation.Semantic proximity Read ↗

Mutation screening of the codon 3500 region of the apolipoprotein B gene by denaturing gradient-gel electrophoresis.Semantic proximity Read ↗

Denaturing gradient-gel electrophoresis screening of familial defective apolipoprotein B-100 in a mixed Asian cohort: two cases of arginine3500-->tryptophan mutation associated with a unique haplotype.Semantic proximity Read ↗

Evaluation of the Roche Diagnostics LightCycler-Apo B 3500 Mutation Detection Kit.Semantic proximity Read ↗

Application of PCR site-directed mutagenesis for a rapid and accurate detection of mutation 3500 (Arg-->Gln) of human apolipoprotein B-100.Semantic proximity Read ↗

Familial defective apolipoprotein B-100. Comparison with familial hypercholesterolemia in 18 cases detected in Munich.Semantic proximity Read ↗

Human fibrinogen polymorphic site analysis by restriction endonuclease digestion and allele-specific polymerase chain reaction amplification: identification of polymorphisms at positions A alpha 312 and B beta 448.Semantic proximity Read ↗

Detection of familial defective apolipoprotein B-100 among patients clinically diagnosed with heterozygous familial hypercholesterolemia in maritime Canada.Semantic proximity Read ↗

A rapid and reliable method for direct genotyping of codon 360 in the human apolipoprotein A-IV gene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.