Article
High photobiological hydrogen production activity of a Nostoc sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity.
Marine biotechnology (New York, N.Y.) - 1 Jan 2000
Yoshino Fuminori, Ikeda Hiroshi, Masukawa Hajime, Sakurai Hidehiro
Abstract excerpt
We describe a strategy to establish cyanobacterial strains with high levels of H(2) production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Culture Media
- DNA, Bacterial
- Gene Expression Regulation, Enzymologic
- Gene Order
- Genes, Homeobox
- Hydrogen
- Hydrogenase
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Nitrogenase